Au Nanoparticles on 4-Thiophenol-Electrodeposited Carbon Surfaces for the Simultaneous Detection of 8-Hydroxyguanine and Guanine

In this proof-of-concept study, gold nanoparticles (AuNPs) were immobilized on glassy carbon electrode (GCE) surfaces using a surface-anchored diazonium salt of 4-aminothiophenol (GCE-Ph-S-AuNPs). X-ray photoelectron spectroscopy (XPS) studies confirmed the attachment AuNPs via 4-thiophenol onto surface modified electrode. Differential pulse voltammetry (DPV) was performed for simultaneous determination guanine (G) and 8-hydroxyguanine (8-OH-G). The calibration curves linear up to 140 µM 60 with limit detection 0.02 0.021 G 8-OH-G, respectively. Moreover, chronoamperometric carried out diffusion coefficients 8-OH-G G. GCE-Ph-S-AuNPs also applied in genomic DNA-spiked samples recovery rates between 98.5% 103.3%. novel electrochemical provided potential platform sensitive related oxidative stress-induced DNA damage clinical studies.